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Omega products aid in COVID-19 detection

The most common COVID-19 testing protocols utilize fluorescence quenching assays and polymerase chain reaction (PCR). For example, in the TAQman assays (from Roche Diagnostics), there is fluorescence quenching (dark green circle) within the DNA-binding probe specific to a particular DNA sequence (dark blue). This probe can be specific to viral, bacterial or any other specific DNA sequence in a sample. As TAQ-polymerase (purple oval) replicates the single-stranded DNA (light blue), it encounters the labeled probe and cleaves the nucleotides off, releasing the fluorescent moiety (bright green circle).  When excited with blue light, this compound glows green. The fluorescence is proportional to the number of DNA sequences the probe finds in the sample. If the signal is too low after a single polymerization step, the sample can be heated to separate the two strands of DNA and the process is repeated. These machines are often also called thermocyclers because the heating system cycles on with each round of replication. As with all fluorescence methods, filters are required to specifically excite the fluorophores and separate the fluorescence signals from scattered light.

A list of stock filter sets for the common PCR dyes are listed below.

These are 25 mm round for excitation and emission filters and 25.7x36 mm for dichroics designed to work at 45 degrees. We also have extensive plate stock and components available, so contact us if you have special configuration requests or  you don’t see what you’re looking for here.

Fluorophore Ex Max (nm) Em Max (nm) Suggested Filter Set
SYBR green 497 520 XF404
Cy-5 651 670 XF416
FAM 494 520 XF404
JOE 520 548 XF405, XF402
VIC 538 554 XF405, XF402
HEX 535 556 XF405, XF402
NED 546 575 XF405, XF402
PET 556 595 XF402
ROX 575 602 XF424
TAMRA 555 580 XF402
TET 521 536 XF405, XF402
Texas Red 595 615 XF424

 

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Custom Filters Will Differentiate Your Product!

Omega has the expertise and tools to develop a filter that will maximize the performance of your instrument. Our in house optics shop can provide you with custom sizes, shapes and substrates. We offer a variety of filter types including bandpass, narrowband, longpass, shortpass, rejection band, beamsplitters and mirrors in any of the following configurations:

  • Protected & Surface Coatings
  • Wavelengths: 190nm – 2500nm
  • Substrate Size: 2mm– 200mm (ask about larger sizes)
  • Filter Thickness: 0.2 – 50 mm
  • Substrate Materials: BK-7, Borofloat, Fused Silica, Sapphire, Germanium, Silicon, colored glasses

Omega can provide filter expertise for your project at every step of the way. We have a team of engineers that can work with you to determine the best set of specifications to meet your performance and cost goals for your application. We’ve worked with thousands of customers, providing more than 6 million filters in a variety of applications from the prototype stage all the way to high volume production. Whether you need 1, 25, 100 or 5000 filters, we can easily supply the filters you need.

Call us at 18022617300 or email sales@omegafilters.com for a custom quote!

Next Story
Additional Information
Filters for PCR and RT-PCR
Learn more about PCR and RT-PCR
Omega Optical, LLC

21 Omega Drive
Brattleboro, VT 05301
USA
U.S. Toll-Free: 1-866-488-1064
International: +1 (802) 251-7300
Sales and Support: sales@omegafilters.com
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Products listed on the website may be limited to the current inventory. Omega cannot guarantee their availability in the future. Contact us for details, or to request pricing of a custom part.

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